The Cologna laboratory uses state-of-the-art mass spectrometry to better understand human disease. We are focused in three major research areas: (i) protein isoform characterization and quantification by mass spectrometry, (ii) mass spectrometry-based lipid measurements in neurodegenerative diseases and (iii) analytical method development and implementation of novel approaches to improve the detection of biomolecules. The overarching goal of this research is to develop tools which can be implemented to study biological pathways perturbed in human disease. Successful outcomes will lead to improved understanding of molecular and cellular processes and potentially lead to therapeutic options for various disorders.